Avacta Analytical Essay

Optim 2

Optim is an innovative analytical instrument designed to probe multiple protein stability-indicating parameters at high speed using ultra-low sample volumes of proteins and other molecules.   
   
Compact and robust, using a light scattering and fluorescence technology in a configuration never seen before and controlled by an all-new easy-to-use intuitive software package, the Optim 2 has been designed to reduce the time and cost of therapeutic protein preformulation studies, protein stability testing and formulation development.   
   
Optim 2 will simultaneously measure a range of protein stability indicating parameters including unfolding transition temperature (Tm), aggregation onset temperature (Tagg) and rates of aggregation, giving you greater insight into identifying the candidate molecules or formulations with the most favourable properties much earlier in the development process. Earlier analysis eliminates the cost associated with the unnecessary development of candidates or formulations that do not have the potential to proceed and dramatically reduces the chance of late-stage failure.

Optim 2

Depth 50 cm
Height 58 cm
Width 54 cm
Power Requirements 110 – 240 V ac, 50 – 60 Hz
Peak Power 600 W
Weight 46 kg
Additional SpecificationsMinimum sample volume:  
9 Μl 
Simultaneous samples per experiment:  
48 
Heating rate:  
0.01 – 10 °C/minute (relevance is sample dependent) 
Protein concentration range:  
Protein dependent:  
0.1 mg/ml – 150 mg/ml (typical IgG) 
SLS sensitivity:  
12 kDa.mg/ml – 22500 kDa.mg/ml (Dextran and IgG) 
SLS precision:  
70 °C) 
Sample temperature precision:  
± 0.3 °C (SD, 48 identical samples, Tm~42 °C) 
 
Environmental conditions 
Temperature range: 18 – 28 °C 
Humidity: 40 to 60 % relative humidity (non-condensing) 
Environmental conditions must be kept constant to maintain calibration

Features

Innovative features offered by Optim:   
       
  • Simultaneous fluorescence and light scattering analysis for label free probing of both protein unfolding and aggregation   
  • Analysis of the effects of temperature, time and chemical environment on your biomolecule   
  • Capability to analyse up to 48 temperature controlled samples simultaneously   
  • 144 samples measured in a high resolution thermal ramp per day   
  • Best in class sample volumes: only 9 microlitres per sample   
  • Sealed sample chamber to enable high temperature analysis without evaporation   
  • Easy-to use software with exceptional functionality and performance designed around the needs of the protein scientist   
   
Optim 2 is designed to address specific industry requirements:   
       
  • Better prediction of stability characteristics of biopharmaceuticals compared to traditional methods   
  • Probing of multiple protein stability indicating parameters within the same instrument   
  • Increased speed of analysis   
  • Considerably lower sample volumes than existing technologies   
  • Facilitation of decision making earlier in development   
   
Applications   
Optim is designed to measure the aggregation, unfolding and stability of microlitre volumes of candidate biopharmaceutical products under different physical, chemical and formulation conditions. Optim can be applied to:   
       
  • Lead candidate selection and optimisation   
  • Preformulation studies   
  • Candidate formulation   
  • Comparability studies   
  • Stability studies   

Photos

Optim 2

Manuals and Resources

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Instrument Documents

Optim 2 Brochure

Optim 1000

Optim is an innovative analytical instrument designed to probe multiple protein stability-indicating parameters at high speed using ultra-low sample volumes of proteins and other molecules.     
     
The Optim 1000 was developed in collaboration with a leading global biopharmaceutical company and has been designed to reduce the time and cost of therapeutic protein preformulation studies, protein stability testing and formulation development. It simultaneously measures a range of protein stability indicating parameters including unfolding transition temperature (Tm) and aggregation onset temperature (Tagg) thereby identifying the candidate molecules or formulations with the most favourable properties much earlier in the development process. Earlier analysis eliminates the cost associated with the unnecessary development of candidates or formulations that do not have the potential to proceed and dramatically reduces the chance of late-stage failure.

Optim 1000

Depth 69 cm
Height 76 cm
Width 85 cm
Power Requirements 220 – 240 V AC
Weight 75 kg
Additional SpecificationsOptim micro-cuvette array (MCA):   
Single use micro-cuvette array with 16x micro-cuvettes. Instrument accommodates up   
to 3 MCAs (48 samples).   
Available in 1 µl or 9 µl volume versions.   
Micro cuvette pitch compatible with 384 well plates and standard 16 channel pipettes.   
Minimum Protein concentration:    
Protein dependant. For typical IgG < 0.1 mg/ml.   
Detector:   
TE cooled, scientific grade CCD camera, 1024 x 256 pixels.   
Operational wavelength range ~250 – 1000 nm .   
Imaging Spectrograph:   
Achromatic, aspheric optics f/4.0.   
Standard grating with optimised efficiency in UV-blue region of spectrum.   
User selectable centre wavelength.   
Software controlled motorized entrance slit.   
Proprietary Optical Configuration:   
Optimised for simultaneous high quality multi-modal optical measurements from small   
sample volumes.   
Laser source 1:    
266 nm laser to excite intrinsic protein fluorescence and for sensitive light scattering.   
Laser source 2:    
473 nm laser for light scattering and excitation of some dyes.   
Sample temperature control:   
TE control of temperature of all 48 samples simultaneously.   
Temperature range: 10 to 100 °C.   
Temperature accuracy +/- 0.1°C at room temperature, +/- 0.5 °C at 100°C.   
Optim Software:   
Full instrument control.   
Integrated data analysis.   
Database for data storage.   
Data export and auto report generation.   
External Computer:   
Desktop PC supplied pre-loaded with Optim software.   
W x D x H 17 cm x 43 cm x 37 cm, Standard LCD monitor, keyboard and mouse.   
Environmental conditions:   
Temperature range: 18 to 28 °C.   
Humidity: 40 to 60% relative humidity (non-condensing).   
Temperature and humidity should be kept constant during operation.   
Electrical:   
Factory set at one of the following options:   
220–240V AC, 50-60Hz, 8-9 A, 10 A, 2 kW (VA)   
110–125V AC, 50-60Hz, 16-18 A, 20 A, 2 kW (VA)

Features

Innovative features offered by Optim:     
         
  • Simultaneous fluorescence and light scattering analysis for label free probing of both protein unfolding and aggregation     
  • Analysis of the effects of temperature, time and chemical environment on your biomolecule     
  • Capability to analyse up to 48 temperature controlled samples simultaneously     
  • Best in class sample throughput: 144 samples per standard working day     
  • Best in class sample volumes: only 9 microlitres per sample     
  • Sealed sample chamber to enable high temperature analysis without evaporation     
  • User interface designed around the needs of the protein scientist     
     
Optim 1000 is designed to address specific industry requirements:     
         
  • Better prediction of stability characteristics of biopharmaceuticals compared to traditional methods     
  • Probing of multiple protein stability indicating parameters within the same instrument     
  • Increased speed of analysis     
  • Considerably lower sample volumes than existing technologies     
  • Facilitation of decision making earlier in development     
     
Applications     
Optim is designed to measure the aggregation, unfolding and stability of microlitre volumes of candidate biopharmaceutical products under different physical, chemical and formulation conditions. Optim can be applied to:     
         
  • Lead candidate selection and optimisation     
  • Preformulation studies     
  • Candidate formulation     
  • Comparability studies     
  • Stability studies     
  • Process optimisation     

Photos

Optim 1000

Manuals and Resources

Note: We appreciate the support of individuals and companies making their documents and resources available to our community. If you find a document or resource that does not belong please let us know by emailing info@labwrench.com

Instrument Documents

Optim® 1000 Brochure

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